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Electrical signals generated by soil microorganisms in microbial fuel cells respond linearly to soil Cd2+ pollution

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Publication date: October 2015
Source:Geoderma, Volumes 255–256
Author(s): Yun-Bin Jiang , Huan Deng , Dong-Mei Sun , Wen-Hui Zhong
We aimed to determine if electrical signals generated by soil microorganisms in microbial fuel cells (MFCs) can accurately respond to Cd2+ concentrations and to evaluate the linear detection range of the method. MFCs were packed with soil spiked with 10 to 400mgkg1 Cd2+ and operated for 37h at constant temperature (30°C). The generated coulomb (C) and start-up time (ST) of the MFCs were calculated as electrical signals. The dehydrogenase activity (DHA) of the Cd2+-spiked soil was determined as a reference method. After MFC operation, the diversity of anodic bacteria was studied by using PCR-DGGE and the sequencing of 16S rRNA genes. The results showed that with increasing Cd2+ concentration, C decreased while ST increased significantly. In the control, C was 30.86±3.84C and ST was 14.39±1.44h; these values decreased to 8.29±5.08C and 30.39±2.87h, respectively, for the treatment with 400mgkg1 Cd2+. Both C (r2 =0.943, P <0.01) and ST (r2 =0.937, P <0.01) were linearly related to Cd2+ at concentrations ranging from 10 to 100mgkg1 and were in agreement with DHA. Cadmium stress significantly decreased the Shannon diversity and changed the community structure of anodic bacteria. Phylogenetic analysis of DGGE bands showed that the anodic bacteria were mainly affiliated with Bacillus, Comamonas, Clostridium and Enterobacter. We propose that electrical signals can be used to evaluate the toxicity of Cd2+ to soil microorganisms at concentrations below 100mgkg1.


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