Seasonal changes in microbial activity in a semiarid soil after application of a high dose of different organic amendments

Publication date: September 2013
Source:Geoderma, Volume 206
Author(s): Ignacio Jorge-Mardomingo , Pedro Soler-Rovira , Miguel Ángel Casermeiro , María Teresa de la Cruz , Alfredo Polo
The application of organic wastes as amendments to improve soil properties has become a very common practice, especially under Mediterranean semiarid conditions. We investigated changes in soil microbial activity under field conditions over a one-year period after the application of a single high dose (160Mgha⁻¹ dry mass) of three organic amendments subjected to different stabilization processes: a municipal solid waste compost (MSWC), and aerobically (AES) and anaerobically digested (ANS) sewage sludge. Measurements were made for microbial biomass carbon (MBC), basal respiration (BR) and metabolic quotient (MQ), and enzymatic activities evaluated by assays of catalase (CA), dehydrogenase (DA), urease (UA), protease (PA), phosphatase (PhA) and β-glucosidase (βGA). These organic amendments produced different effects on soil microbial activity depending on the treatment and stabilization processes of the organic wastes. The application of MSWC significantly increased (p ≤0.05) the MBC, with the highest content observed in summer season (1369.1±13.2mgCkg⁻¹). Soil microbial activity (BR, CA, DA and hydrolase activity) remained stable throughout the one-year period in MSWC. In soils amended with sewage sludges the content of MBC did not increase although shortly after application a significant rise (p ≤0.05) was observed for BR and MQ. The highest BR values were 23.88±13.2 and 9.28±0.81μgO2 g⁻¹ h⁻¹ for AES and ANS, respectively. While MQ was increased to 38.05±1.8 and 18.21±0.76ngO2 μg⁻¹ MBCh⁻¹, in AES and ANS respectively. Moreover, AES and ANS treatments led to different patterns in soil enzyme activity. In the short-term both treatments increased the oxidoreductase enzyme activity, with maximum of CA in AES (53.6±0.82μmolO2 g⁻¹ min⁻¹) and DA in ANS (7.2±0.4mgINTFg⁻¹ h⁻¹). Also in the initial stage they enhanced the activity of hydrolases like PA in ANS (0.72±0.01μmolNH4 ⁺ g⁻¹ h⁻¹) and PhA in AES (156±6.6μmolPNPg⁻¹ h⁻¹). Our results indicate that the type of stabilization of the organic amendments determines soil microbial activity, which responds differently depending on the type of organic material added.