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Sampling designs for soil organic carbon stock assessment of soil profiles

Publication date: 1 December 2017
Source:Geoderma, Volume 307
Author(s): Yakun Zhang, Alfred E. Hartemink
We investigated four sampling designs for soil organic carbon (SOC) stock assessment of soil profiles: (i) sampling by horizons, (ii) vertical transect sampling, (iii) depth-based stratified random sampling, (iv) fuzzy c-means sampling in which we explored the use of vis-NIR spectroscopy, image analysis and color models. An Alfisol and Mollisol profile wall (1×1m) was divided into a 10×10cm raster and 100 samples (about 200g each) were collected at the centers of grid cells for SOC analysis. Bulk density samples were collected from each 10-cm depth interval along a single vertical transect and the SOC stock was calculated using 100 points in the profile wall. Horizon-based sampling for the Mollisol (5 horizons) ranged from 231 to 262MgCha1, whereas it ranged from 69 to 99MgCha1 in the Alfisol (3 horizons). The SOC stocks obtained by 1 to 7 vertical transects ranged from 68 to 81MgCha1 in the Alfisol, and 239 to 246MgCha1 in the Mollisol. Depth-based stratified random sampling resulted in the SOC stocks ranging from 77 to 82MgCha1 in the Alfisol and 234 to 257MgCha1 in the Mollisol, and the standard errors decreased with increasing sample size from 10 to 70. Fuzzy c-means clustering created clusters similar to the field delineated horizons. A sample size of 7 in both profiles was sufficient to estimate the mean profile SOC stock by fuzzy c-means sampling. The CIE L*a*b* color model resulted in more accurate estimation in the Alfisol, but the vis-NIR spectra resulted in more accurate estimation in the Mollisol. Soil depth improved the performance of vis-NIR spectra. It is concluded that in these soils, at least two or three vertical transects are required to capture the horizontal variation for estimating profile SOC stock. Depth-wise stratified random sampling reduces the number of samples and is suitable when horizontal variation is high. Fuzzy c-means sampling is useful to determine the minimum sample size for profile SOC stock assessment but requires ancillary data and processing before sampling the soil profile.


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